Maintaining your hplc uhplc uplc systems.
Waters hplc system cleaning procedure.
Water flush to clear customer s mobile phases from the system.
This sop shall be applicable for the hplc system waters alliances 2690 2695 separation modules.
Use solvent inlet filters to protect the system from incoming particles.
Responsibility quality control officer executive.
The water flushes are only needed to remove the previous solvent from the system.
To clearly lay down the procedure for cleaning.
Controlling contamination in lc ms and hplc ms systems 715001307.
Use only hplc grade solvents and water filtered through 0 2.
3 0 responsibility 3 1 doing.
Disconnect the column and attach a union and flow directly to waste.
Place a b c and d seal wash and needle wash lines into a clean.
Technical assistant 3 2 checking.
Reverse osmosis to remove most contaminants b.
Water flush to clean acid from the system.
Ion exchange to remove any remaining ions.
For information on other acquity uplc cleaning methods refer to the recommended cleaning mixtures for lc table in the con trolling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
When preparing a system to run your method it is always important to prime up the system to ensure you get fresh solvents in all of the required lines.
Label bottles correctly with bottle content and filling date expiry date.
To clean the system.
Cleaning mixtures for lc table in the controlling contamination in ultraperformance lc ms and hplc ms systems document p n 715001307.
Ultrapure water is water that has been purified through a system that targets contami nants detrimental to uplc ms and hplc ms systems.
When working with any lc system setting it up and storing it is very important.
To clean the system.
Executive manager 4 0 accountability head of the department 5 0 procedure 5 1 for system cleaning.
Water acetonitrile methanol ipa 1 1 1 1 with 2 5 fa.
10 nh4oh in water this removes peg and other.
Cleaning decontamination columns general guidelines flow cells general.
Flush the system using one of the cleaning mixtures as described in the cleaning solution table of the controlling contamination in uplc ms and hplc ms sytems guide 715001307.
Objective to lay down the procedure for operation of hplc waters alliance 2695 separation module scope to ensure that the instrument performs satisfactory and gives accurate and reproducible data.
It is necessary to restore the system flow path to a near new level of cleanliness note.
The purification process should include all of the following steps.
No injections are needed with that.
